Explore the seamless journey from whole blood to functional cells with pluriSpin Human PLT Depletion, a method reshaping cell isolation through one-step negative separation grounded in density gradient centrifugation.
In the realm of cell isolation, the precision and efficiency of the process are critical to obtaining functional cells for various applications. PluriSpin Human PLT Depletion emerges as a beacon of innovation, offering a seamless isolation process designed to tackle the persistent challenge of platelet contamination. Utilizing a one-step negative cell separation technique grounded in density gradient centrifugation principle, this method not only eliminates platelets but also enhances the purity and viability of the enriched cell population. Let’s delve into the intricacies of pluriSpin’s Human PLT Depletion, exploring its solutions to platelet-related issues and the advantages it brings to the forefront.
The Platelet Predicament: Challenges and Solutions
Challenges with Platelet Contamination
Platelet contamination poses multifaceted challenges that can compromise the purity and functionality of isolated cells, especially when working with fresh blood or samples older than 8 hours. The issues range from a reduction in the purity of enriched cells to platelet-induced disturbances in cell cultures, clumping, and interference with analytical techniques like FACS analysis.
Solutions and Advantages with pluriSpin Human PLT Depletion
Enter pluriSpin Human PLT Depletion, a solution meticulously crafted to address these challenges. Through a depletion process devoid of beads, this method employs density gradient centrifugation in a single step, offering a unique set of advantages:
Enhanced Purity through Density Gradient Centrifugation
PluriSpin’s approach involves a one-step negative cell separation technology using density gradient centrifugation, significantly boosting the purity of enriched cells. The method efficiently removes unwanted platelets along with red blood cells (RBCs), ensuring that the isolated cell population remains pristine.
Compatibility with Negative Separation Techniques
PluriSpin Human PLT Depletion can seamlessly integrate with other negative separation techniques, such as pluriSpin CD45 Depletion, further enhancing rare cell enrichment capabilities. This versatility empowers researchers to tailor their cell isolation strategies based on specific experimental requirements.
Reduced Manipulation with Shorter Centrifugation Steps
The method minimizes the manipulation of blood samples by employing a shorter centrifugation step compared to standard protocols with platelet density gradients. This not only saves time but also contributes to maintaining the integrity of the isolated cells.
Improved Cell Enrichment from Older Blood Samples
PluriSpin’s innovative process demonstrates effectiveness in improving cell enrichment from blood samples older than 8 hours, presenting a valuable solution for scenarios where sample age might be a limiting factor.
Unpacking the Facts & Features of pluriSpin Human PLT Depletion
Affordable One-Step Negative Cell Separation
PluriSpin Human PLT Depletion streamlines the cell separation process into an affordable, single step, making it an economical choice for laboratories with varying budgets.
Independent of RBC Concentration
The method remains independent of red blood cell concentration, ensuring consistent performance across different sample compositions.
Grounded in Density Centrifugation
Leveraging density centrifugation principles, pluriSpin’s approach stands as a reliable and scientifically sound method for cell isolation.
Untouched Cells for Maximum Viability
By avoiding the use of beads and magnets, pluriSpin Human PLT Depletion ensures that the isolated cells remain untouched, preserving their viability for downstream applications.
No Columns, No Magnets, No Special Equipment
The simplicity of the pluriSpin method shines through its equipment-independent design. No columns or magnets are required, and no specialized training or equipment is necessary, making it accessible to researchers across various expertise levels.
Isolate from Various Sample Sources
PluriSpin’s versatility extends to sample sources, allowing researchers to isolate cells from whole blood, buffy coat, or cord blood with equal efficacy.
Remove Platelet Contamination with PLT Depletion
The primary objective of pluriSpin Human PLT Depletion is clear: to effectively remove platelet contamination, ensuring a pure and functional cell population for downstream applications.
How It Works: A Seamless Journey from Whole Blood to Functional Cells
Label:
Add pluriSpin (50 μl per 1 ml sample). The pluriSpin suspension efficiently binds unwanted cells during this labeling step.
Incubate:
Allow the sample to incubate for 15 minutes on a pluriPlix or rolling mixer. This phase promotes optimal binding of unwanted cells to the pluriSpin suspension.
Layer over Density Gradient Medium:
The labeled sample is layered over a density gradient medium, setting the stage for the subsequent centrifugation process.
Spin:
Centrifuge the sample for 15 minutes at 800 x g, with the brake off. The density gradient centrifugation separates unwanted cells and platelets, allowing the purified cell population to collect at the interface.
Collect the Enriched Cells:
Once centrifugation is complete, the enriched cell population is ready for collection. The simplicity of this step ensures a straightforward retrieval process.
Wash:
A final wash step guarantees the removal of any remaining impurities, leaving behind a pristine, functional cell population ready for downstream applications.
Conclusion: Seamless Isolation with Density Gradient Centrifugation
In the intricate landscape of cell separation, pluriSpin Human PLT Depletion stands as a testament to innovation, offering a seamless isolation process that addresses the challenges posed by platelet contamination. Through the elegant integration of density gradient centrifugation, this method not only removes unwanted platelets efficiently but also enhances the purity, viability, and functionality of the isolated cell population.
pluriSpin’s approach to cell separation signifies a paradigm shift in the quest for functional cells. By navigating through the challenges associated with platelet contamination, this method paves the way for researchers to confidently move from whole blood to functional cells, opening new avenues for exploration in cellular research. In the evolving narrative of laboratory techniques, pluriSpin Human PLT Depletion takes its place as a reliable and efficient tool, contributing to the advancement of cell isolation methodologies grounded in scientific rigor and practicality.
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