Gentle Mouse B Cell Enrichment: How to Culture Isolated Fragile Mouse B Cells

This blog is about how pluribead helps in the isolation of B cells from single-cell suspensions of splenocytes or other tissues. Keep reading to learn more.

The production of antibodies is the main role of B cells. They are known as B cells because, in contrast to T cells, which mature in the thymus, B cells develop in the bone marrow. A B cell’s surface receptors bind to foreign antigens to produce antibodies that suppress a particular pathogen. The functioning of B cells can be better understood by learning more about them.

B cells must be separated from neighboring cell populations and preserved in order to be studied properly. B cells can be more difficult to gather and isolate than other leukocytes due to their rarity and fragility. A large number of human B cells would be ideal to study, but occasionally this is not the case, and researchers must look elsewhere for human B cells.

Want to know more about how Pluribead cascade straining helps in Mouse B Cell Enrichment?

Mouse Cell Separation vs. Human Cell Separation

When conducting immunological experiments, murine cells are frequently used in place of human cells. There are many differences between mice and humans despite the fact that there are only about 300 genes that are different in the two species, according to recent genomic studies. Both innate and adaptive immune responses begin to function in different ways. Certain components of the human immune response do not manifest in mice. Experiments may not produce results that are accurate for modeling human responses if these variations are not taken into consideration.

Different cell samples can affect the process of cell separation in addition to variations in performance. Human blood, for instance, contains a lot of neutrophils. About 70% to 30% of lymphocytes and neutrophils are in balance. In mice, lymphocytes take on a greater weight than neutrophils, with about 80% lymphocytes and 20% neutrophils. This may make it more difficult for researchers to separate out particular cell populations from one tissue or another.

In order to obtain a sufficient, healthy sample from mouse tissue for neutrophil research, may prove difficult. This is one of the main justifications for the popularity of mouse lymphocyte enrichment. Some murine organs are excellent candidates for cell separation because they contain large numbers of T and B cells.

Mouse B Cell Enrichment vs. Mouse T Cell Enrichment

There are various types of murine lymphocytes that can be specifically discussed. There are differences between the B cell and T cell populations in mouse organs. The spleen is one organ in mice that contains a lot of lymphocytes. About 25% of the leukocytes in the mouse spleen are T cells, and 50% of them are B cells (the remaining 25% are neutrophils, monocytes, dendritic cells, etc.).

Research on human immunology can benefit from using murine T and B cells. To obtain the largest sample possible when isolating mouse B cells, it is beneficial to focus on organs with high concentrations, like the spleen.

Gentle Mouse B Cell Enrichment Strategy

Although mouse B cells can be found in higher concentrations in some areas, this does not mean that they are always available. The body contains various types of B cells at various times, and occasionally researchers may be searching for B cells that have been activated in a particular manner. This makes it crucial to exercise caution when enriching a B cell population. The throughput of cell separation assays is increased while cell health and physiology are preserved using gentle mouse B cell enrichment techniques.

Pluribead for Gentle Mouse B Cell Enrichment

Many conventional mouse B cell enrichment techniques have been reported to cause harm to delicate cell populations and to be expensive to execute. Methods like flow cytometry and centrifugation rely on harsh physical forces to sort cells, which could result in shearing and ultimately more cell death. The magnetic field in magnetic-based cell separation techniques can cause cells to lyse. The gentle mouse B cell enrichment using Pluribead preserves cell viability throughout the isolation procedure.

Pluribead

When the specific antibody binds directly to the cells using positive selection, all undesirable cells are separated from the labeled and desired cells during the subsequent enrichment steps because they are all unbound. When coupled to a solid phase, a cell strainer is the simplest way to keep labeled cells in place. Any type of sample material, such as PBMC, secretion or excretion material, whole blood, buffy coat, spleen, liver, and so on, may be used.

Two Different Bead Sizes are Available

S-pluriBead: It is used in large sample volumes with a small number of targets.
M-pluriBead: A versatile material that can be used to achieve multiple goals while using less material.

Key features of Pluribead

No Sample Preparation: Use a sample volume ranging from 200 l to 45 ml with no erythrolysis, gradient centrifugation, or other techniques.
Any type of sample material, such as PBMC, secretion/excretion material, brain homogenate, spleen, liver, buffy coat, whole blood, and so on, can be used.
Suitable for Isolation from a Wide Range of Species: Isolate from sheep, mice, rats, cows, dogs, and other animals.
Simultaneous Cell Isolation using PluriBead Cascade straining: Separate two different cell types from the same sample material at the same time.
Using sequential cell isolation, you can isolate up to six different targets from a single sample.

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Reference:

Nature

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