The cells will be “touched” by the antibody. All the unwanted cells remain unbound and will be separated from the labeled and wanted cells in the following enrichment steps. The easiest way to hold back the labeled cells, when coupled to a solid phase, is to use cell strainer or magnets. The flow through is free of antibody-bound beads and can be used for another positive or negative cell enrichment. The enrichment with pluriBead can be done without any preparation of whole blood. The contamination with unbound cells especially platelets or erythrocytes is very low when the cells are directly isolated out of whole blood. The method fast, less expansive and results in a higher purity.
GF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing domain.
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