UberStrainer Question and Answer

pluriBead® is a non-magnetic cell separation technology that uses antibody-coated beads and a strainer-based workflow to isolate target cells. Unlike magnetic separation systems, pluriBead® does not require magnetic equipment, making cell isolation simpler while still delivering high-purity results.

Yes. One of the key advantages of pluriBead® technology is that it can be used directly with whole blood and many other sample types without gradient centrifugation, erythrocyte lysis, or extensive sample preparation. This helps save time and preserve cell viability.

pluriBead® is compatible with a wide range of sample materials, including whole blood, buffy coat, PBMCs, brain homogenates, spleen tissue, liver tissue, secretions, excretions, cell cultures, and more. It can also be used for the isolation of proteins, viruses, bacteria, and nucleic acids.

S-pluriBead® is typically recommended for isolating small numbers of target cells from larger sample volumes, such as circulating tumor cells (CTCs). M-pluriBead® is better suited for isolating larger numbers of target cells from smaller sample volumes, such as buffy coat preparations.

pluriBead® offers rapid isolation starting from as little as five minutes, high cell viability, minimal sample preparation, and low contamination from unwanted cells. It also supports sequential and simultaneous isolation of multiple targets, making it a flexible and cost-effective solution for a variety of research applications.

pluriSpin® is a negative cell separation system designed to isolate highly purified, untouched target cells by removing unwanted cell populations. It is commonly used for enriching monocytes, T cells, CD4⁺ T cells, CD8⁺ T cells, NK cells, B cells, and other cell types from whole blood, buffy coat, or cord blood samples.

Unlike magnetic separation systems, pluriSpin® does not require magnets, columns, or specialized equipment. The target cells remain free of antibodies and beads, helping preserve cell viability and functionality while minimizing the risk of cell activation or damage.

Yes. pluriSpin® is designed to work directly with whole blood, buffy coat, and cord blood samples. The system is based on density gradient centrifugation and does not require extensive sample preparation before cell isolation.

Negative selection leaves the desired cells untouched while removing unwanted cells. This approach helps maintain natural cell characteristics, improves cell viability, and produces enriched cell populations that are ready for downstream applications such as cell culture, functional assays, and flow cytometry.

No. Laboratories already familiar with density gradient centrifugation can easily incorporate pluriSpin® into their workflows. The process requires no special training, magnets, or columns, making it a cost-effective and straightforward solution for cell enrichment and depletion applications.

TwinSpin® is a density gradient centrifugation system designed for the enrichment and isolation of specific cell populations from whole blood, bone marrow, buffy coat, and cord blood. It simplifies sample preparation and enables efficient recovery of target cells for downstream research applications.

TwinSpin® combines density gradient separation and cell collection in a single device. After centrifugation, the inner tube can be removed, and the integrated dropper-style collection system allows researchers to recover enriched cells with minimal handling and reduced risk of disturbing the cell layer.

The cell population isolated depends on the density gradient medium used. Options include:
- Leuko Spin Medium for all leukocytes
- Lympho Spin Medium for PBMCs
- Lympho Spin 24+ Medium for PBMCs from older blood samples
- PLT Spin Medium for platelets
This flexibility allows researchers to select the most appropriate medium for their specific application.

Yes. TwinSpin® can be paired with Lympho Spin 24+ Medium, which is specifically designed for blood samples that are 8 to 48 hours old. This helps improve PBMC recovery and reduce contamination from granulocytes and debris in aged samples.

TwinSpin® minimizes sample handling, reduces cell stress, eliminates the need for delicate layer collection procedures, and supports high cell viability and yield. It also works with standard density gradient centrifugation protocols and requires no specialized training or equipment, making it an efficient and user-friendly solution for cell enrichment workflows.

pluriMate® separation tubes are designed for the efficient isolation of leukocytes and peripheral blood mononuclear cells (PBMCs) from whole blood, buffy coat, cord blood, and bone marrow. They simplify density gradient centrifugation by eliminating the need for manual sample layering.

The proprietary porous polyurethane barrier prevents the sample material from mixing with the density gradient medium during setup. After centrifugation, it also helps prevent recontamination of the enriched cell fraction, resulting in cleaner cell recovery and more consistent results.

pluriMate® tubes are designed for use with whole blood, buffy coat, cord blood, and bone marrow samples. They are not intended for other sample types outside these applications.

pluriMate® is available in 2 mL, 15 mL, and 50 mL formats to accommodate different sample volumes. The appropriate size depends on the amount of sample being processed, allowing laboratories to efficiently handle both small and large-volume applications.

pluriMate® reduces hands-on preparation time, eliminates the need for careful sample overlaying, minimizes the risk of sample mixing, and helps improve cell recovery. Its user-friendly design makes density gradient centrifugation faster, more reproducible, and easier to perform, even for routine laboratory workflows.

Leuko Spin Medium is a sterile, ready-to-use density gradient medium designed for the isolation and enrichment of all leukocytes from fresh human peripheral blood or buffy coat samples. It enables the recovery of both mononuclear and polymorphonuclear cell populations in a single centrifugation step.

Leuko Spin Medium is designed to enrich all leukocytes, including peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells such as granulocytes. This makes it suitable for applications requiring a broad leukocyte population.

Yes. Leuko Spin Medium can be used with pluriMate® separation tubes to simplify sample preparation and reduce the risk of mixing between the sample and density gradient medium. This can help improve workflow efficiency and cell recovery.

Cells enriched with Leuko Spin Medium can be used in a variety of research applications, including magnetic cell sorting (MACS), flow cytometry, cell culture, immunology studies, stem cell research, and other cell-based analyses.

Leuko Spin Medium is specifically formulated to achieve high-yield enrichment of both mononuclear and polymorphonuclear leukocytes in a single step. Its ready-to-use format, compatibility with pluriMate® tubes, and ability to support multiple downstream applications make it a convenient and reliable choice for cell isolation workflows.

PBMC Spin Medium is a sterile, ready-to-use density gradient medium designed for the isolation and enrichment of peripheral blood mononuclear cells (PBMCs) from fresh human whole blood or buffy coat samples. It provides high-yield recovery of mononuclear cells for research and laboratory applications.

Yes. PBMC Spin Medium can be used as an alternative to commonly used PBMC isolation media without requiring significant changes to existing density gradient centrifugation protocols. This allows laboratories to transition easily while maintaining familiar workflows.

PBMC Spin Medium is designed to enrich mononuclear cells, including lymphocytes and monocytes. During centrifugation, erythrocytes, granulocytes, and dead cells pass through the density medium, allowing PBMCs to collect at the plasma-density medium interface for easy recovery.

PBMCs isolated with PBMC Spin Medium are suitable for a wide range of applications, including flow cytometry, magnetic cell sorting (MACS), pluriBead® cell isolation, immunology research, cell culture studies, and stem cell research.

PBMC Spin Medium offers a high-yield, single-step isolation process, consistent performance, and a ready-to-use formulation. It can also be combined with pluriMate® separation tubes for simplified sample handling, making it a convenient and reliable solution for PBMC enrichment workflows.

PBMC Spin 24+ Medium is a sterile, ready-to-use density gradient medium specifically designed for the isolation of peripheral blood mononuclear cells (PBMCs) from blood or buffy coat samples that are 8 to 24 hours old. It helps maintain high cell purity and recovery when processing aged samples.

PBMC Spin 24+ Medium is optimized for older blood samples. It helps reduce the contamination of PBMC fractions with granulocytes and erythrocytes that commonly increases as blood samples age, resulting in cleaner cell preparations and improved recovery.

PBMC Spin 24+ Medium is recommended when blood or buffy coat samples cannot be processed immediately and are between 8 and 24 hours old. It is an ideal solution for laboratories receiving shipped samples or working with delayed processing schedules.

PBMC Spin 24+ Medium is designed to provide high-purity PBMC enrichment with yields of up to 80%, even when processing samples up to 24 hours after collection, helping maximize the value of precious specimens.

PBMCs isolated with PBMC Spin 24+ Medium can be used for flow cytometry, magnetic cell sorting (MACS), immunology studies, cell culture, stem cell research, and other cell-based applications that require high-quality mononuclear cell populations.

PLT Spin Medium is a sterile, ready-to-use density gradient medium designed for the isolation and enrichment of platelets from fresh human peripheral blood. It enables efficient platelet recovery through a simple, single-step centrifugation process.

PLT Spin Medium is specifically formulated to achieve high-yield platelet enrichment while minimizing contamination from unwanted blood components. Its ready-to-use format helps simplify laboratory workflows and reduce preparation time.

Following density gradient centrifugation, the enriched platelet fraction is found at the plasma-density medium interface and within the upper layer above the PLT Spin Medium, allowing for straightforward collection and processing.

Yes. PLT Spin Medium is compatible with pluriMate® separation tubes, which help prevent sample mixing during setup and simplify the recovery of enriched platelets after centrifugation.

Platelets enriched with PLT Spin Medium can be used for a variety of downstream applications, including platelet biology studies, cell signaling research, hemostasis research, biomarker analysis, and other platelet-related laboratory investigations.

Monocytes Spin Medium is a sterile, ready-to-use density gradient medium designed for the enrichment of monocytes from fresh peripheral blood or buffy coat samples. It enables high-yield monocyte isolation through a single-step density centrifugation process.

Depending on the sample quality and donor variability, Monocytes Spin Medium can achieve monocyte purities of approximately 60–80%. This represents a significant enrichment compared to standard PBMC isolation methods.

Unlike conventional PBMC density media, which enrich a mixed population of mononuclear cells, Monocytes Spin Medium is specifically optimized to increase the proportion of monocytes in the recovered cell fraction, helping reduce the need for additional enrichment steps.

Monocytes Spin Medium provides a gentle, density-based enrichment method that does not require magnetic beads, antibodies, or labeling. This helps preserve natural cell characteristics and makes it particularly suitable for cell culture, differentiation studies, and other sensitive downstream applications.

Enriched monocytes can be used for cell culture, macrophage and dendritic cell differentiation, immunology research, cytokine studies, functional assays, flow cytometry, and other applications requiring viable monocyte populations.

High Density Spin Medium is a sterile, ready-to-use solution designed for preparing custom density gradient media. It allows researchers to create specific density working solutions for the enrichment and separation of cells, bacteria, and other particles based on their experimental requirements.

High Density Spin Medium offers greater flexibility by allowing users to prepare customized density gradients. This is particularly useful for specialized applications where standard density media may not provide the desired separation performance.

Custom density solutions are prepared by mixing High Density Spin Medium with Density Diluent Medium in the appropriate ratio. The final density can be adjusted to suit specific target cells or particles, such as monocytes, PBMCs, or polymorphonuclear cells.

High Density Spin Medium can be used to prepare density solutions for the enrichment of a wide range of targets, including human monocytes, PBMCs, polymorphonuclear cells (PMNCs), bacteria, and other biological or particulate samples.

Yes. High Density Spin Medium is highly viscous and should be pipetted slowly to ensure accurate measurements. Both the High Density Spin Medium and Density Diluent Medium should be at the same temperature before mixing, and the solution should be stirred gently to avoid introducing air bubbles that could affect density gradient performance.

Density Diluent Medium is a sterile, ready-to-use solution designed for preparing custom density gradient media. It is used in combination with High Density Spin Medium to create density working solutions for the enrichment and separation of cells, bacteria, and other particles.

Density Diluent Medium allows researchers to accurately adjust the density of High Density Spin Medium to achieve the optimal separation conditions for specific cell types or particles. This flexibility helps improve isolation efficiency and purity.

Density Diluent Medium can be used to prepare customized density gradients for a variety of applications, including the enrichment of PBMCs, monocytes, granulocytes, bacteria, and other biological particles that require density-based separation.

Yes. Density Diluent Medium is supplied sterile and ready to use. It only needs to be mixed with High Density Spin Medium in the appropriate ratio to create the desired density solution for your application.

Yes. Because it is designed for creating customized density solutions, Density Diluent Medium can support a wide range of density gradient centrifugation workflows, making it a versatile choice for laboratories working with different cell types and particle separation requirements.