Why do we use it?

pluriStrainer

PET vs. Nylon mesh – Which Material suits me?

With our pluriStrainers, we offer two different materials for a selection of mesh sizes – PET and nylon.

To help you decide which material is most suitable for your application, we have summarised the most important properties of the PET and nylon mesh below:

Both materials are suitable without restrictions if you are working with aqueous solutions or media in the physiological pH range (e.g. wash buffer, cell culture medium, PBS, HBSS, RBC lysis buffer).

If the cells, spheroids, tissue or other sample material are to be mechanically disrupted before flow through the pluriStrainer, the PET mesh should be chosen because of its higher strength and stability.

If you are planning a phenol-chloroform extraction on the pluriStrainer, we recommend the PET mesh (an incubation period of 15min showed no limitations for the quality of the DNA, RNA or proteins in our tests).

If you use acetone, the nylon mesh is recommended because of its better resistance.

Both materials have suitable stability against ethanol.

If you are working with more concentrated acids or bases, you should choose PET mesh for acids and nylon mesh for basic solutions.

Please note:

The information on stability is a guideline and does not exclude individual application. Material stability also depends on the duration of contact. A short contact with a chemical may have no effect even on a more sensitive mesh material, so that both PET and nylon can be considered for your application.

For the PET mesh, you have a wider range of mesh sizes available. Therefore, if the pore size of the pluriStrainer mesh is the relevant factor for you, you will usually find the right one for you in our pluriStrainers with PET mesh!

PET

Nylon

Aqueous liquids

in physiological pH ranges (e.g. wash buffer, cell culture media, PBS, HBSS, RBC Lysis Buffer)

✔

No restrictions known

✔

No restrictions known

Phenol-Chloroform (e.g. for DNA extraction on the strainer)

✔

Limited resistance

but suitable for short-term contact (Tested for DNA, RNA and protein extraction for 15 min incubation time with proper results)

Limited resistance

Not tested

Acetone resistance

Limited resistance

Short-term contact is possible if necessary

✔

Good

Ethanol resistance

✔

Good

✔

Good

Acid resistance

✔

Good

for most of solutions used in laboratory

Limited

more restrictions on the solutions used in the laboratory

Alkaline resistance

Limited

more restrictions on the solutions used in the laboratory

✔

Good

for most of solutions used in laboratory

Mechanical disruption

(e.g. grinding of cells, cell cluster, spheroids, tissue, plant material)

✔

Good

Robust, stiff

Limited

Soft, flexible

Maximum working temperature dry

150°C

115°C

Cell Separation

Particle Separation

All-Star Strainer

Lab Essentials

Recent Posts

Archives

Recent Posts

Archives

Related Articles

TwinSpin – A Device for Gravity Separation

Why do we use it?

Circulating Tumor Cells (CTC)

Pluriselect’s All-Star-Strainer-Team: One Stop Solution to Particle Separation

A Detailed Guide on Positive Selection vs Negative Selection for Cell Isolation

T Cell Isolation :A Comprehensive Guide to the Key Components

What is Buffy Coat in Blood? Buffy Coat Preparation and Buffy Coat Cell Extraction

Necrosis Vs. Apoptosis: Necrotic Cell Death, Processes, & Apoptosis Steps